Enzymatic Synthesis of Acetyl Phosphate*
نویسنده
چکیده
In order to prove this suggestion, a study of the chemistry of acetyl phosphate was undertaken and eventually a method of determination and a simple synthesis were worked out (3). With these available, on oxidation of pyruvate the appearance of a labile organic phosphate was observed (4) which closely resembled acetyl phosphate. The present, communication reports upon t.he isolation of this intermediary and its identification with acetyl phosphate. The second part of the paper deals with the linking between pyruvate oxidation and adenylic acid phosphorylation through acetyl phosphate as the intermediary. This transfer of the phospholyl group between acetic and adenylic acid was found to be reversible.
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